ORIGINAL RESEARCH
Year : 2012 | Volume
: 3 | Issue : 1 | Page : 610--614
Antibacterial activity of the crude extract of Azadirachta Indica on Enterococcus Faecalis: A comparative in vitro study
Anil Pulipparambil1, Sai Sathya Narayana2, Meyyappan3, Anand S Prabhu4, Gurucharan5, Arun Kulandaivelu2
1 Senior lecturer, Department of Conservative Dentistry and Endodontics, Malabar Dental College and Hospital, Kuttipuram, Kerala, India
2 Reader, Department of Conservative Dentistry and Endodontics, RMDC&H, Chidambaram, Tamilnadu, India
3 Professor, Department of Conservative Dentistry and Endodontics, RMDC&H, Chidambaram, Tamilnadu, India
4 Senior Lecturer, Department of Pediatric Dentistry, RMDC&H, Chidambaram, Tamilnadu, India
5 Professor and Head, Department of Conservative Dentistry and Endodontics, RMDC&H, Chidambaram, Tamilnadu, India
Correspondence Address:
Dr. Anil Pulipparambil
Senior lecturer, Department of Conservative Dentistry and Endodontics, Malabar Dental College and Hospital, Kuttipuram, Kerala
India
Context: The importance of cleaning & shaping, medication and obturation of the root canal to the success of endodontic procedures is unquestionable. However an underemphasized phase is irrigation. Intracanal cleaning and disinfecting procedures are highly dependent on the mechanical and chemical effects of the irrigants. Aims: The aim of present study was to compare the root canal disinfection by 3% Sodium hypochlorite (NaOCl), 2% Chlorhexidine (CHX) and ethanolic extract of Azadirachta indica. Settings and Design: A comparative invitro study was conducted using human mandibular premolar teeth that were extracted for orthodontic reasons. Methods and Material: Crown of each tooth was removed at cemento enamel junction and root canals were prepared using crown down technique. Each canal was completely filled up with the E. faecalis suspension and irrigated with the test solutions. After serial dilutions, samples were plated onto Pfizer selective Enterococcus agar and the colony forming units were counted both before and after irrigation. Statistical analysis: Results were analyzed by student paired �SQ�t�SQ� test and one-way ANOVA with the level of significance set at P < 0.05. Results: Azadirachta Indica extract exhibited significant resistance to E. Faecalis colonization similar to NaOCl & CHX. Conclusions: Azadirachta indica extract have shown promising results when used as a root canal irrigant. Further studies are required based on tissue-dissolving properties, dentin wall cleaning ability and biocompatibility before specific clinical recommendations can be made. Key Messages: Azadirachta indica, popularly known as neem and considered a cynosure of modern medicine may play a defining role in root canal disinfection.
How to cite this article:
Pulipparambil A, Narayana SS, Meyyappan, Prabhu AS, Gurucharan, Kulandaivelu A. Antibacterial activity of the crude extract of Azadirachta Indica on Enterococcus Faecalis: A comparative in vitro study.Indian J Multidiscip Dent 2012;3:610-614
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How to cite this URL:
Pulipparambil A, Narayana SS, Meyyappan, Prabhu AS, Gurucharan, Kulandaivelu A. Antibacterial activity of the crude extract of Azadirachta Indica on Enterococcus Faecalis: A comparative in vitro study. Indian J Multidiscip Dent [serial online] 2012 [cited 2023 Dec 1 ];3:610-614
Available from: https://www.ijmdent.com/article.asp?issn=2229-6360;year=2012;volume=3;issue=1;spage=610;epage=614;aulast=Pulipparambil;type=0 |
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