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Year : 2015  |  Volume : 5  |  Issue : 1  |  Page : 2-9

Histomorphometric and histological evaluations of the simvastatin effect on alveolar bone loss induced by cyclosporine A in rats

1 Department of Oral Biology, Faculty of Dentistry, Medical Research Institute, Alexandria University, Alexandria, Egypt
2 Department of Histochemistry and Cell Biology, Medical Research Institute, Alexandria University, Alexandria, Egypt

Correspondence Address:
Samir Hagar
Department of Oral Biology, Faculty of Dentistry, Alexandria University, Alexandria
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2229-6360.163640

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Background: Cyclosporin-A- has been used as an immunosuppressant to prevent the rejection of organ transplants. However, alveolar bone loss is an important negative side-effect of this drug. Simvastatin, a hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor, is known to inhibit cholesterol biosynthesis. It has advanced effects on bone formation in vivo and in vitro. So,we evaluated the histological and histomorphometric analysis of osteoblast and osteoclast cells after administration of simvastatin in cyclosporin-A-associated alveolar bone loss in rats. Aim of the Study: To evaluate the effect of simvastatin and cyclosporin -A- on Alveolar bone by investigating the histological and histomorphometric results of osteoblast and osteoclast cells. Materials and Methods: 24 adult male rats will be divided into 3 groups: Group I: Control group; 4 rats, Group II: cyclosporine -A- group; 10 rats (10 mg/kg) subcutaneous injection, Group III: cyclosporine -A-/simvastatin group; 10 rats, simvastatin will be taken orally daily (20mg/kg/day). Two rats from the control group and 5 rats from each of the studied experimental groups (group II & III) were sacrificed on days 15 and 30 consecutively using Histological and Histomorphometric investigations. Results: Histological results revealed higher bone volume and osteoblast cells, and decreased number of osteoclast cells in Simvastatin group than in CsA group. The same results was statistically significant in Histomorphometric results of both osteoblast and osteoclast cells counts. In Histomorphometrical analysis showed a significant increase of osteoblast cells in Simvastatin group than CsA group, and significant decrease of osteoclast cells in Simvastatin group than CsA group. Conclusion: We can conclude that Simvastatin counteract the adverse effect of CsA induced alveolar bone loss that induced new bone formation.

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