Antibacterial activity of the crude extract of Azadirachta Indica on Enterococcus Faecalis: A comparative in vitro study

Anil Pulipparambil; Sai Sathya Narayana; Meyyappan; Anand S Prabhu; Gurucharan; Arun Kulandaivelu

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ORIGINAL RESEARCH

Year : 2012 | Volume : 3 | Issue : 1 | Page : 610-614

Antibacterial activity of the crude extract of Azadirachta Indica on Enterococcus Faecalis: A comparative in vitro study

Anil Pulipparambil¹, Sai Sathya Narayana², Meyyappan³, Anand S Prabhu⁴, Gurucharan⁵, Arun Kulandaivelu²
¹ Senior lecturer, Department of Conservative Dentistry and Endodontics, Malabar Dental College and Hospital, Kuttipuram, Kerala, India
² Reader, Department of Conservative Dentistry and Endodontics, RMDC&H, Chidambaram, Tamilnadu, India
³ Professor, Department of Conservative Dentistry and Endodontics, RMDC&H, Chidambaram, Tamilnadu, India
⁴ Senior Lecturer, Department of Pediatric Dentistry, RMDC&H, Chidambaram, Tamilnadu, India
⁵ Professor and Head, Department of Conservative Dentistry and Endodontics, RMDC&H, Chidambaram, Tamilnadu, India

Correspondence Address:
Dr. Anil Pulipparambil
Senior lecturer, Department of Conservative Dentistry and Endodontics, Malabar Dental College and Hospital, Kuttipuram, Kerala
India

Source of Support: None, Conflict of Interest: None

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Context: The importance of cleaning & shaping, medication and obturation of the root canal to the success of endodontic procedures is unquestionable. However an underemphasized phase is irrigation. Intracanal cleaning and disinfecting procedures are highly dependent on the mechanical and chemical effects of the irrigants. Aims: The aim of present study was to compare the root canal disinfection by 3% Sodium hypochlorite (NaOCl), 2% Chlorhexidine (CHX) and ethanolic extract of Azadirachta indica. Settings and Design: A comparative invitro study was conducted using human mandibular premolar teeth that were extracted for orthodontic reasons. Methods and Material: Crown of each tooth was removed at cemento enamel junction and root canals were prepared using crown down technique. Each canal was completely filled up with the E. faecalis suspension and irrigated with the test solutions. After serial dilutions, samples were plated onto Pfizer selective Enterococcus agar and the colony forming units were counted both before and after irrigation. Statistical analysis: Results were analyzed by student paired 't' test and one-way ANOVA with the level of significance set at P < 0.05. Results: Azadirachta Indica extract exhibited significant resistance to E. Faecalis colonization similar to NaOCl & CHX. Conclusions: Azadirachta indica extract have shown promising results when used as a root canal irrigant. Further studies are required based on tissue-dissolving properties, dentin wall cleaning ability and biocompatibility before specific clinical recommendations can be made. Key Messages: Azadirachta indica, popularly known as neem and considered a cynosure of modern medicine may play a defining role in root canal disinfection.

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