CASE REPORT |
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Year : 2011 | Volume
: 2
| Issue : 1 | Page : 411-414 |
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Bordetella avium and bacillus megaterium in endodontic infection
Arunajatesan Subbiya1, Krishnan Mahalakshmi2
1 Professor, Dept. of Conservative Dentistry and Endodontics, Sree Balaji Dental College and Hospital, Chennai, India 2 Associate Professor, Dept. of Microbiology, Sree Balaji Dental College and Hospital, Chennai, India
Correspondence Address:
Dr. Krishnan Mahalakshmi Associate Professor, Dept. of Microbiology, Sree Balaji Dental College and Hospital, Velachery-Tambaram Road, Chennai - 600 100, Tamil Nadu India
Source of Support: None, Conflict of Interest: None | Check |
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Many microbiological studies on endodontic infections indicate a complex polymicrobial community. Different identification methods used have revealed a diverse microflora in the endodontic niche. Recently 16S rRNA/DNA gene sequencing method of identification is widely employed in dentistry which has discovered novel pathogens that may be uncultivable or possibly slow growing and difficult to identify. In the present study, nested polymerase chain reaction (PCR) was performed with the endodontic samples of the two patients with diffuse swelling and pain near the region of tooth with prosthetic crown as these two samples were culture negative. 16S rRNA universal eubacterial primers were used for rapid identification. Unusually, the organisms identified in both the cases were of single etiology. Bordetella avium was identified in the endodontic sample of a tooth with prosthetic crown in a 56-year-old woman and Bacillus megaterium in a 65-year-sold man. The occurrence of B. avium and B. megaterium on the teeth may be correlated to their ability to secrete collagenase. Direct screening of the clinical samples by molecular approach has identified unusual human bacterial pathogens in tooth with prosthetic crown. In addition, the results of this study also reveal that endodontic infection need not be polymicrobial all the time. Screening of bacterial pathogens in the endodontic samples may help in treatment planning and treatment evaluation. |
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